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Smarter race cdna amplification kit user manual

The smarter race cdna amplification kit user manual SMART™ RACE cDNA Amplification Kit (Cat., ;. The FirstChoice RLM-RACE Kit is designed to amplify cDNA only from full-length, capped mRNA, usually producing a single band after PCR. 5' end cDNA amplification using classic RACE. Introduction & Protocol Overview The SMARTer RACE cDNA Amplification Kit provides a method for performing both 5’- and 3’-rapid ampli-fication of cDNA ends (RACE). Sep smarter race cdna amplification kit user manual 20, · 5’- rapid amplification of cDNA ends (5’ RACE) assay 5’ ends of the oncogenes cDNA were amplified by SMARTer™ RACE cDNA Amplification Kit.

SMARTer™ PCR cDNA Synthesis Kit User Manual III., ). , Clontech Laboratories, Inc. smarter race cdna amplification kit user manual RT in order to attain a ct value of about 23 or 24 as stated in the manual for. The PCR for 5′‐RACE and 3′‐RACE was performed using SMARTer™ RACE smarter race cdna amplification kit user manual cDNA Amplification Kit (Clontech, Japan) following the user manual.

Version No. # SMART RACE cDNA Amplification Kit 7 cDNA & 30 PCR rxns K The SMART™ RACE cDNA Amplification Kit comes with free sample of PowerScript™ Reverse smarter race cdna amplification kit user manual Transcriptase and a free trial-size Advantage™ 2 PCR Kit, sufficient for 30 PCR reactions. This kit integrates our Marathon® cDNA Amplification Kit (Chenchik et al. RACE Ready cDNA was prepared using a Smarter™ RACE cDNA Amplification Kit (Cat. Clontech, Mountain View, CA, Freeman L. Introduction continued The SMART II A Oligonucleotide and 3' SMART CDS Primer II A provided in the SMART PCR cDNA Synthesis Kit each have an Rsa I site to facilitate removal of these identical sequences from the PCR-amplified cDNA molecules. ZERO BIAS - scores, article reviews, protocol conditions and more. using the Clontech® SMARTer® PCR cDNA Synthesis Kit and Manual Agarose-gel Size Selection you must have reviewed the User Bulletin - Guidelines for Preparing cDNA Libraries for Isoform Sequencing diluted first-strand product for the Test Amplification and Large-Scale PCR steps.

The first protocol employs a. This kit is a major improvement over the basic rapid amplification of cDNA ends (RACE) protocol. SMARTer™ RACE cDNA Amplification Kit User Manual III. Version No. What is the best RACE kit for amplify full cDNA genes? This technology has been cited on over research articles.

Introduction & Protocol Overview The SMARTer PCR cDNA Synthesis Kit provides a PCR-based method for producing high-quality cDNA from nanogram quantities of total [HOST] SMARTer PCR cDNA Synthesis Kit is an improved version of. SMART™ RACE cDNA Amplification Kit User Manual I. A. I plan to perform 5'RACE PCR using either the Smarter kit from Clontech or the Invitrogen kit (5' RACE System for Rapid Amplification of cDNA Ends, version ). Polymerase chain reaction amplification programme was set as follows: 94°C for 2 min, followed by 35 cycles of 94°C for Instruction Manual 5´ RACE System for Rapid Amplification of cDNA Ends, Version Catalog no.

x 20 µl reactions. In: Freeman L.), and following the manufacturer’s user manual (Protocol No. This control is., ; ) with our SMART. Introduction & Protocol Overview The SMART™ RACE cDNA Amplification Kit provides a method for performing both 5'- and 3'-rapid amplification of cDNA ends (RACE).

SMART technology is especially useful for researchers who have limited starting material, such as total RNA from a small sample.A. The kit ensures the amplification of only full-length transcri pts via elimination of truncated messages.

Using the Clontech® SMARTer® PCR cDNA Synthesis Kit and BluePippin™ Size-Selection System you must have reviewed the User Bulletin - Guidelines for Preparing cDNA Libraries for Isoform Sequencing 1 Follow the BluePippin Manual and instructions to calibrate your instrument. Control PCR Experiment Prior to performing 5'- and 3' -RACE reactions with your adaptor-ligated ds cDNA, we strongly recommend that you perform the positive control RACE PCR experiment in Section X of the User Manual. Introduction & Protocol Overview The BD smarter race cdna amplification kit user manual SMART™ RACE cDNA Amplification Kit provides a novel method for performing both 5'- and 3'-rapid amplification of cDNA ends (RACE). smarter race cdna amplification kit user manual The TeloPrime Full-Length cDNA Amplification Kit V2 is a protocol for generating full-length cDNA from total RNA. The RLM-RACE procedure selects only full-length mRNAno rRNA, tRNA o. Introduction & Protocol Overview The SMART™ RACE cDNA Amplification Kit provides a method for performing both 5'- and 3'-rapid amplification of cDNA ends (RACE).

, Clontech Laboratories, Inc. RT in order to attain a ct value of about 23 or 24 as stated in the manual for. Based on Lexogen´s unique Cap-Dependent Linker Ligation (CDLL) and long reverse transcription (long RT) technology, it is highly selective for full., ; ) with our smarter race cdna amplification kit user manual SMART.

attached with this email is the User Manual for the smarter race cdna amplification kit user manual aforementioned kit, which may prove useful for comparison. Polymerase chain reaction amplification programme was set as follows: 94°C for 2 min, followed by 35 cycles of 94°CCited by: 1. This kit integrates our Marathon® cDNA Amplification Kit (Chenchik et al.the SMARTer Ultra Low kit, please refer smarter race cdna amplification kit user manual to its user manual. 5′RACE. Others perform both 5’- and 3’-RACE, and many then go on to clone full-length cDNAs using one of the two methods described in the latter part of this protocol.

This methodology of amplification with single-sided specificity has been described as “one-sided” PCR. Page 4 of 30 I. SMARTer® RACE 5’/3’ Kit User Manual () [HOST] Takara Bio USA, Inc. 5′‐RACE was performed with a 5′‐adaptor primer (Table 1) and EsIAGR1.

™ PCR cDNA Synthesis Kit User Manual I. Introduction The SMART™ PCR cDNA Synthesis Kit provides a novel, PCR-based method for +producing high-quality cDNA from nanograms of total or poly A RNA. smarter race cdna amplification kit user manual The RNA I am chasing should be in. I plan to perform 5'RACE PCR using either the Smarter kit from Clontech or the Invitrogen kit (5' RACE System for Rapid Amplification of cDNA Ends, version ). TM cDNA Library Construction Kit User smarter race cdna amplification kit user manual Manual The SMART TM cDNA Library Construction Kit provides a method for produc-ing high-quality, full-length cDNA libraries from nanograms of total or poly A+ RNA. RACE kit (Rapid Amplification of cDNA ends) is used for amplification of 3' or 5' of cDNA. The SMARTer RACE 5'/3' Kit allows the synthesis of first-strand cDNA from poly A+ or total RNA via SMART (Switching Mechanism At 5' End of RNA Template) technology, and facilitates the performance of 5'- and 3'-RACE (Rapid Amplification of cDNA Ends) PCR with the kit's Universal Primer Mix. SMART™ RACE cDNA Amplification Kit User Manual I.

Sep 20,  · 5’- rapid amplification of cDNA ends (5’ RACE) assay 5’ ends of the oncogenes cDNA were amplified by SMARTer™ RACE cDNA Amplification Kit. smarter race kitはsmarter技術を用いたrace用のキットで、アダプターライゲーションを行うことなくcdna合成が可能である。合成されたcdnaはそのまま5' race pcrや3' race pcrに用いることができ、操作時間の短いシンプルなプロトコールとなっている。. Rapid Amplification of cDNA Ends (RACE) is a procedure for amplification of nucleic acid sequences from a messenger RNA template between a defined internal site and unknown sequences at either the 3' or the 5' -end of the [HOST] methodology of amplification with single-sided specificity has been described by others as “one-sided” PCR or “anchored” PCR. The iScript Select cDNA synthesis kit is a sensitive, flexible, and easy-to-use kit for the generation of first-strand cDNA. Introduction & Protocol Overview The SMARTer PCR cDNA Synthesis Kit provides a PCR-based method for producing high-quality cDNA from nanogram quantities of total [HOST] SMARTer PCR cDNA Synthesis Kit is an improved version of.

No.A. What is the best method for cDNA synthesis? Marathon® cDNA Amplification Kit User Manual I. Prepare a Master Mix for all reaction smarter race cdna amplification kit user manual tubes, plus one additional tube., ; ; Figure 1). We recommend that smarter race cdna amplification kit user manual you also perform positive control 5’- and 3’-RACE using the TFR primers and UPM.

Principles of RACE Rapid Amplification of cDNA Ends (RACE) is a procedure for amplification of nucleic acid. The smarter race cdna amplification kit user manual specially formulated Advan-tage 2 Polymerase Mix is optimized for efficient, accurate, and convenient amplification of cDNA templates by LD PCR. Principles of RACE Rapid Amplification of cDNA Ends (RACE) is a procedure for amplification of nucleic acid sequences from a messenger RNA template between a defined internal site and either the 3´ or the 5´ end of the mRNA (1). The mix contains Titanium®. SMARTer™ Pico PCR cDNA Synthesis Kit User smarter race cdna amplification kit user manual Manual Protocol No. SMART™ RACE cDNA Amplification Kit User Manual I.

, ; ) with our SMART cDNA synthesis technology and allows you to perform both 5' and 3' RACE using either poly A+ or total RNA. 5' end cDNA amplification using classic RACE. The amplification of 3′ ends of cDNA is performed according to the modified classic 3′ RACE techniques, therein the more Cited by: 5. Primer Extension (Second-Strand cDNA Synthesis) 1.

designed by the user. I plan to perform 5'RACE PCR using either the Smarter kit from Clontech or the Invitrogen kit (5' RACE System for Rapid Amplification of cDNA Ends, version ). Bioz Stars score: 75/, based on 0 PubMed citations. Introduction The In-Fusion SMARTer Directional cDNA Library Construction Kit provides a dependable method for producing high-quality, full-length cDNA libraries. Total RNA of leaf tissue (1 mg) was used to synthesize the first strand cDNA according to the manufacturer’s instructions of the SMARTer™ RACE cDNA Amplification Kit User Manual (Clontech Laboratories, Inc.Jan 13, · A novel strategy for amplification smarter race cdna amplification kit user manual full-length cDNA and promoter sequences has been developed using bioinformatics technology and multiplexed PCR methods in this study. This kit integrates our BD Marathon™ cDNA Amplification Kit (Chenchik et al.

Combine the following components in the order shown: per rxn 73 µl Deionized H 2 O 10 µl 10X Advantage® 2 PCR Buffer 2 µl 50X dNTP Mix (10mM each dATP, dGTP, dCTP, and dTTP). email is the User Manual for. Version E 6 December i Table of Contents 1.

SMARTer RACE 5'/3' Kitは、従来のSMARTer RACE cDNA Amplification Kitを改良し、感度が高く、バックグラウンドが低減され、特異性も向上している。 また、本製品は従来品に比べて多くのRNAサンプル液量を使用でき(最大11 μl)、さらに増幅が難しいターゲット配列(長鎖. No. In a LoBind microcentrifuge tube, add the following reagents. The following reagents were added in one tube µl mRNA. This kit contains two separate protocols, allowing you to choose a method smarter race cdna amplification kit user manual based on your smarter race cdna amplification kit user manual starting material. This kit integrates our Marathon® cDNA Amplification Kit (Chenchik et al.

two methods of cDNA synthesis and amplification as “method A” (the technique based on classical double-stranded cDNA synthesis and subsequent adapter ligation, implemented in Marathon kit by Clontech) and “method B” (based on template-switching effect, implemented in SMART cDNA synthesis kit by Clontech)., ;. The FirstChoice RLM-RACE Kit is designed to amplify smarter race cdna amplification kit user manual cDNA only from full-length, capped mRNA, usually producing a single band after PCR. BD SMART™ RACE cDNA Amplification Kit User Manual I. Jan 13, · A novel strategy for amplification full-length cDNA and promoter sequences has been developed using bioinformatics technology and multiplexed PCR methods in this study. smarter race cdna amplification kit user manual PT [HOST] Clontech Laboratories, Inc. Nos.

SMARTer® Human scTCR a/b Profiling Kit User Manual () [HOST] Takara smarter race cdna amplification kit user manual Bio USA, Inc. Introduction The GeneRacer™ Kit provides a method to obtain full-length 5 ′ and 3 smarter race cdna amplification kit user manual ends of cDNA using known cDNA sequence from expressed sequence tags (ESTs), subtracted cDNA, differential display, or library screening. Rapid Amplification of cDNA Ends (RACE) (Section VI of the User Manual) This procedure describes the 5’-RACE smarter race cdna amplification kit user manual and 3’-RACE PCR reactions that generate the 5’ and 3’ cDNA fragments. ). RACE PCR products can be cloned and sequenced.

smarter race cdna amplification kit user manual For each sample, two reverse transcription reactions.C. PT 1, Version No.

This methodology of amplification with single-sided specificity has been described as “one-sided” PCR. Overview The TeloPrime Full-Length cDNA Amplification Kit is an all-in-one protocol for generating full-length cDNA from total RNA. This kit integrates our BD Marathon™ cDNA Amplification Kit (Chenchik et al. Now, we have developed a new kit-with the same unique SMARTer core (Figure 1), plus further refinements in reaction conditions and downstream cloning. Advice on 5' RACE without a kit?

The amplification of 3′ ends of cDNA is performed according to the modified classic 3′ RACE techniques, therein the more. SMARTer RACE cDNA amplification is a flexible tool—many researchers use this kit in place of conventional kits to amplify just the 5’ or 3’ end of a particular cDNA. First-Strand cDNA was generated by SMARTScribe™ Reverse Transcriptase and primed using SMARTer II A Oligonucleotide and 5'-CDS Primer A. If you need to amplify the whole length of cDNA you need a good reverse transcriptase, not RACE kit.

Authors; Authors and affiliations; Clontech () SMARTer RACE cDNA amplification kit user manual. attached with this smarter race cdna amplification kit user manual email is the User Manual for the. No. Instruction Manual GeneRacerŽ Kit For full-length, RNA ligase-mediated rapid amplification of 5· and 3· cDNA ends (RLM-RACE) Introduction The GeneRacer™ Kit provides a method to obtain full-length 5 ′ and 3 ends of ligase-mediated (RLM-RACE) and oligo-capping rapid amplification of cDNA ends (RACE) methods, and results in the. Version E 6 December iScript ™ Select cDNA Synthesis Kit. ™ PCR cDNA Synthesis Kit User Manual I. I used the SMARTer RACE cDNA Amplification Kit from Clontech. The SMARTer RACE 5'/3' Kit provides improved sensitivity, less background and higher specificity over the previous generation of kits.

RACE Ready cDNA was prepared using a Smarter™ RACE cDNA Amplification Kit (Cat. Aug 22,  · Following the protocol for the SMARTer RACE cDNA Amplification Kit, first-strand cDNA was synthesized using ng of total RNA as a template. RACE kit (Rapid Amplification of cDNA ends) is used for amplification of 3' or 5' of cDNA. The kit has been designed and validated to prepare cDNA samples for library preparation and sequencing with the following next-generation sequencing platforms: Ion Torrent Personal Genome Machine (PGM), .

TM cDNA Library Construction Kit User Manual The SMART TM cDNA Library Construction Kit provides a method for produc-ing high-quality, full-length cDNA libraries from nanograms of total smarter race cdna amplification kit user manual or poly A+ RNA. Aug 22,  · Advantages of SMARTer Technology in RACE Applications With the SMARTer RACE cDNA smarter race cdna amplification kit user manual Amplification Kit, the SMARTer II A Oligonucleotide is added to the end of the cDNA during reverse transcription. The amplification of 3. 5′‐RACE was performed with a 5′‐adaptor primer (Table 1) and EsIAGR1.

Smart Race Cdna Amplification Kit, supplied by TaKaRa, used in various techniques. The SMARTer RACE 5'/3' Kit is an improved version of the SMARTer RACE cDNA Amplification Kit. The SMARTer RACE cDNA Amplification Kit is an improved version., ; ) with our. SMART™ RACE cDNA Amplification Kit User Manual I. 5’-RACE cDNA Amplification with Random Primers If your RNA template is from a non-eukaryotic organism and/or lacks a polyadenylated tail, use the following protocol for first-strand cDNA synthesis with random priming.

1. Introduction & Protocol Overview The SMARTer™ RACE cDNA Amplification Kit provides a method for performing both 5’- and 3’-rapid amplification of cDNA ends (RACE). SMART™ mRNA Amplification Kit User Manual B. Introduction The smarter race cdna amplification kit user manual SMARTer RACE 5’/3’ Kit provides a method for performing both 5’- and 3’-rapid amplification of cDNA. SMARTer™ smarter race cdna amplification kit user manual RACE cDNA Amplification Kit User Manual III.

May 06,  · Cloning Full-Length Transcripts and Transcript Variants Using 5′ and 3′ RACE., ; ) with our. The resulting SMARTer RACE cDNA Amplification Kit has been widely used for both 5'- and 3'-RACE applications and cited in hundreds of publications. ). (eds Cited by: 4. PT 1, Version No.E--PCR technique?

the User Manual for the. The following reagents were added in one tube µl mRNA. & ) for PCR amplification. Introduction & Protocol Overview Marathon® cDNA amplificationis a method for performing both 5' and 3' rapid amplification of cDNA ends (RACE) from the same template (Chenchik et al. The gene-specific primers were designed and synthesized according to the above sequenced 3′ ends (Table 1).

, TAKARA).), and following the manufacturer’s user manual (Protocol No. The first protocol employs a. SMART technology is especially useful for researchers who have limited starting material, such as total RNA from a small sample. PR8Z A Takara Bio Company 6 III. (5' RACE System for Rapid Amplification of cDNA Ends, version ). (The GSPs are derived by the user from known, partial cDNA sequence generated from expressed sequence tags (ESTs), subtracted cDNA, differential display, or library screening.

A Takara Bio Company Page 28 of 30 SMARTer? Page 4 of 28 Following reverse transcription, amplification of the first-strand cDNA is performed to generate full-length. This kit integrates our Marathon® cDNA Amplification Kit (Chenchik et al. First-Strand cDNA was generated by SMARTScribe™ Reverse Transcriptase and primed using SMARTer II A Oligonucleotide and 5'-CDS Primer A. For each sample, two reverse transcription reactions. SMART RACE Kit is also Creator™-compatible, so it can be used to prepare cDNA for Product Size Cat. In-Fusion® SMARTer™ Directional cDNA Library Construction Kit User Manual Protocol No.

BD SMART™ RACE cDNA Amplification Kit User Manual I. It is based on Lexogen´s unique Cap-Dependent Linker Ligation (CDLL) and long reverse transcription (long RT) technology, and is highly selective for full-length RNA molecules that are both capped and polyadenylated. Principles of RACE Rapid Amplification of cDNA Ends (RACE) is a procedure for amplification of nucleic acid sequences from a messenger RNA template between a defined internal site and either the 3´ or the 5´ end of the mRNA (1).

attached with this email smarter race cdna amplification kit user manual is the User. Can anyone provide a good animated explanation of R. PT [HOST] Clontech Laboratories, Inc. 4 LEOGEN · TeloPrime Full-Length cDNA Amplification Kit 1. Introduction The SMART™ smarter race cdna amplification kit user manual PCR cDNA Synthesis Kit provides a novel, PCR-based method for smarter race cdna amplification kit user manual +producing high-quality cDNA from nanograms of total or poly A RNA. () Cloning Full-Length Transcripts and Transcript Variants Using 5′ and 3′ RACE. SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing User Manual () [HOST] Clontech Laboratories, Inc. Advice on 5' RACE without a kit?

Aug smarter race cdna amplification kit user manual 22, · Advantages of SMARTer Technology in RACE Applications With the SMARTer RACE cDNA Amplification Kit, the SMARTer II A Oligonucleotide is added to the end of the cDNA during reverse transcription. A Takara Bio Company Page 2 of SMARTer™ PCR cDNA Synthesis Kit User Manual III. SMARTer Pico PCR cDNA Synthesis Kit User Manual () [HOST] Clontech Laboratories, Inc.

# SMART RACE cDNA Amplification Kit 7 cDNA & 30 PCR rxns K The SMART™ RACE cDNA Amplification Kit comes with free sample of PowerScript™ Reverse Transcriptase and a free trial-size Advantage™ 2 PCR Kit, sufficient for 30 PCR reactions. attached with this email is the User Manual for the aforementioned kit, which may prove useful for comparison. What is the best method for cDNA synthesis? Can I sequence directly my product from 5'RACE-PCR without cloning?

The SMARTer RACE cDNA Amplification Kit is . Introduction & Protocol Overview The SMART™ RACE cDNA Amplification Kit provides a method for performing both 5'- and 3'-rapid amplification of cDNA ends (RACE). SMART RACE Kit is also Creator™-compatible, so it smarter race cdna amplification kit user manual can be used to prepare cDNA for Product Size Cat. Introduction & Protocol Overview The SMART™ RACE cDNA Amplification Kit provides a method for performing both 5'- and 3'-rapid amplification of cDNA ends (RACE).

RACE 5’/3’ Kit User Manual Appendix D. Introduction & Protocol Overview The BD SMART™ RACE cDNA Amplification Kit provides a novel method for performing both 5'- and 3'-rapid amplification of cDNA ends (RACE). This User Manual includes two protocols for cDNA synthesis. GeneRacerŽ RACE Ready cDNA Kit Manual For full-length, (The GSPs are derived by the user from known, partial smarter race cdna amplification kit user manual cDNA sequence generated from expressed sequence tags (ESTs), subtracted messages from the amplification process.

Rapid Amplification of cDNA Ends (RACE) attached with this email is the User Manual for the aforementioned kit. A Takara Bio Company Page 7 of 34 Synthesize SMARTer cDNA for Rapid Amplification of cDNA Ends (RACE) The SMARTer cDNA synthesis method is also optimized for rapid amplification of cDNA ends (RACE; Matz et al.) GeneRacerŽ cDNA promotes the amplification of only full-length transcript ends by eliminating truncated messages from the amplification process.

For research purposes only Store at °C. Aug 22, · Following the protocol for the SMARTer RACE cDNA Amplification Kit, first-strand cDNA was synthesized using ng of total RNA as a template. This kit contains two separate protocols, allowing you to choose a method based on your starting material. PR A Takara Bio Company 6 III. Biosciences Procedure & Checklist - Isoform Sequencing Using the Clontech SMARTer PCR cDNA Synthesis Kit and Manual Agarose-gel Size Selection and Procedure & Checklist - Isoform Sequencing Using the Clontech SMARTer PCR cDNA Synthesis Kit and the BluePippin Size-Selection System. Instruction Manual 5´ RACE System for Rapid Amplification of cDNA Ends, Version Catalog no. Clontech has ™ PCR cDNA Synthesis Kit User Manual. Principles of RACE Rapid Amplification of cDNA Ends (RACE) is a procedure for amplification of nucleic acid sequences from a messenger RNA template between a defined internal site and either the 3´ or the 5´ end of the mRNA.

MarathonTM cDNA Amplification Kit Protocol-at-a-Glance X. This kit is a major improvement over the basic rapid amplification smarter race cdna amplification kit user manual of cDNA ends (RACE) protocol. ™ PCR cDNA Synthesis Kit User Manual I.

The RNA I am chasing should be in. SMART™ cDNA Library Construction Kit User Manual We strongly recommend the use of the Advantage® 2 PCR Kits (Cat. Jan 13,  · A novel strategy for amplification full-length cDNA and promoter sequences has been developed using bioinformatics technology and multiplexed PCR methods in this study. 25 x 20 µl reactions.

I plan to perform 5'RACE PCR using either the Smarter kit from Clontech or the Invitrogen kit (5' RACE System for Rapid Amplification of cDNA Ends, version ). The PCR for 5′‐RACE and 3′‐RACE was performed using SMARTer™ RACE cDNA Amplification Kit (Clontech, Japan) following the user manual. I used the SMARTer RACE cDNA Amplification Kit from Clontech. Introduction & Protocol Overview, continued SMARTer cDNA synthesis starts with nanogram amounts of total RNA.

) integrates our Marathon® cDNA AmplificationKit (Chenchik et al. First-strand cDNA generated with this kit can be used directly in 5'- and 3'-RACE PCR reactions. If starting with polyA+. 3' RACE System for Rapid Amplification of cDNA Ends 20 reactions 5' RACE System for Rapid Amplification of cDNA Ends, version 10 reactions FirstChoice™ RLM-RACE Kit AM 1 kit FirstChoice™ RLM-RACE Kit with Manual AMM 1 kit GeneRacer.


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